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Image Search Results
Journal: bioRxiv
Article Title: High-throughput protein characterization by complementation using DNA barcoded fragment libraries
doi: 10.1101/2024.05.08.593210
Figure Lengend Snippet: Complementation of CysA activity by Bacillus subtilis CysP and by novel sulfate transporter TK06_RS10770 from Pseudomonas fluorescens FW300-N2E2. First panel shows the native E. coli system. Second panel shows the function of the sulfate permease (CysP) from B. subtilis , along with its AlphaFold predicted structure. The final panel shows the AlphaFold predicted structure for TK06_RS10770 of P. fluorescens FW300-N2E2.
Article Snippet: Genome fragment library generation cloning was carried out in
Techniques: Activity Assay
Journal: bioRxiv
Article Title: High-throughput protein characterization by complementation using DNA barcoded fragment libraries
doi: 10.1101/2024.05.08.593210
Figure Lengend Snippet: Identification of a protein, LRK54_RS05660 of R. denitrificans FW104-10B01 that complements Δ metB knockout. The top of the figure shows the two dominant pathways from homoserine to homocysteine used by bacteria, the two step (MetBC) and one step (MetZ) pathways. The lower portion of the figure shows crystal structure of E. coli MetB and the AlphaFold predicted structure for LRK54_RS05660, which bears structural similarity.
Article Snippet: Genome fragment library generation cloning was carried out in
Techniques: Knock-Out, Bacteria
Journal: bioRxiv
Article Title: High-throughput protein characterization by complementation using DNA barcoded fragment libraries
doi: 10.1101/2024.05.08.593210
Figure Lengend Snippet: HisC activity from TK06_RS12685 of Pseudomonas fluorescens FW300-N2E2. One the left is the AlphaFold predicted structure of TK06_RS12685. On the right is the activity of HisC from E. coli that it is being complemented.
Article Snippet: Genome fragment library generation cloning was carried out in
Techniques: Activity Assay